Fusion proteins of non-attenuated HIV regulatory/accessory proteins

US7569228B2 · kind B2 · utility

Assignee

• Bavarian Nordic A/S · DK

Inventors

• Paul Howley · Scone, AU
• Sonja Leyer · Munich, DE
• Eva Felder · Munich, DE

Key dates

• Filing date: Jul 18, 2006
• Grant date: Aug 4, 2009
• Expiry date: Jul 18, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/16334
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Nucleic acids are disclosed encoding fusion proteins comprising the amino acid sequence of at least four non-attenuated HIV proteins selected from the group consisting of Vif, Vpr, Vpu, Vpx, Rev, Tat, and Nef, wherein the fusion protein does not contain a specific cleavage sequence for a cellular protease. Also disclosed are vectors comprising the nucleic acids and methods of preparing the fusion protein by transfecting a host cell with the nucleic acids or with the vectors containing the nucleic acids, expressing the fusion proteins, and recovering the fusion proteins.