Patent · US Expired

Use of flow-cytrometric analysis to optimize cell banking strategies for CHO cells

US7569339B2 · kind B2 · utility

7Cited by

0References

5Claims

0Family size

Assignee

BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG · DE

Inventors

Hitto Kaufmann · Ulm, DE
Juergen Fieder · Unterstadion, DE
Ralf Otto · Wiesbaden, DE

Key dates

Filing date	Nov 9, 2005
Grant date	Aug 4, 2009
Priority date	—
Expiry date	Jan 26, 2026

Classification

Technology area (CPC G)Physics
CPC primaryG01N2333/4718
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Production of biopharmaceuticals from CHO cells requires generation of master-, working- and post-production cell banks of high quality, partly under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to the desire to establish production processes that are completely devoid of serum or even any animal components and to ensure robust thaw performance for reliable production. Here we describe a novel strategy employing flow cytometric (FC) analysis of Annexin V-stained cells for high-throughput characterization of CHO cell banks. Our data show that this method enables evaluation of a cryopreservation procedure just 6 h after thaw.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.