Homogeneous luminescence energy transfer bioassay
US7569355B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Jun 9, 2005 |
| Grant date | Aug 4, 2009 |
| Priority date | — |
| Expiry date | Dec 5, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2458/40
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A homogeneous bioassay including a first group labelled with an energy donor and a second group labelled with an energy acceptor, where the donor is a luminescent lanthanide label capable of up-converting a lower-energy excitation to a higher-energy emission, where the acceptor is either a luminescent or a non-luminescent label, and where the increase or decrease, respectively, in energy transfer from the donor to the acceptor resulting from shortening or lengthening of the distance between the labels is measured. The assay is performed in a biological fluid which absorbs radiation in the wavelength range 300 to 600 nm, the measurement is carried out at a wavelength >570 nm, and the donor label, which is excitable at wavelength longer than its emission wavelength, is excited in the wavelength window in which the biological fluid does not essentially absorb the excitation radiation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.