Patent · US Active

Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy

US7569367B2 · kind B2 · utility

2Cited by

11References

14Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Michael Knoll · Aschendorf, DE
Walter Eberle · Biberach an der Riß, DE
Thomas Kirschbaum · Munich, DE

Key dates

Filing date	Sep 1, 2006
Grant date	Aug 4, 2009
Priority date	—
Expiry date	Sep 9, 2026

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/1006
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention deals with a method of preparing nucleic acids, particularly RNA, from a whole blood sample. The nucleic acids purified by the method of the invention are particularly suited for detection of nucleic acid marker molecules. Preferred are markers for the diagnosis of transmissible spongiform encephalopathy (TSE). Such diagnosis is based on the detection, by means of real-time PCR, of certain mRNAs of the TSE-infected organism. Said mRNAs specifically originate as splicing variants and are isolated from whole blood by the method of the invention.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.