Polypeptides and biosynthetic pathways for the production of monatin and its precursors

US7572607B2 · kind B2 · utility

Assignee

• CARGILL, INCORPORATED · US

Inventors

• Paula M. Hicks · Eden Prairie, US
• Sara C. McFarlan · Windom, US
• Timothy W. Abraham · Minnetonka, US
• Douglas C. Cameron · Plymouth, US
• James R. Millis · Kohler, US
• John Rosazza · Iowa City, US
• Lishan Zhao · Carlsbad, US
• David Weiner · Echt, NL

Key dates

• Filing date: Apr 26, 2005
• Grant date: Aug 11, 2009
• Expiry date: Jan 29, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.