DNA joining method
US7575860B2 · kind B2 · utility
8Cited by
8References
55Claims
0Family size
Inventors
Key dates
| Filing date | Mar 7, 2001 |
| Grant date | Aug 18, 2009 |
| Priority date | — |
| Expiry date | Mar 7, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/66
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method to directionally clone any linear template DNA molecule into any linearized vector. The vector ends may be generated from any restriction enzyme cleavage. The method does not require a ligation step nor the use of carefully controlled conditions as is required with methods involving specific exonucleases alone. It has been determined that specific DNA polymerases are able to efficiently join one or more linear DNA molecules sharing ends with appropriate complementation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.