Ligand/binding partner bio-labeling systems
US7575866B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Sep 1, 2005 |
| Grant date | Aug 18, 2009 |
| Priority date | — |
| Expiry date | Feb 14, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to methods and compositions which utilize binding relationships between detector molecules and their ligands for labeling molecules of interest in vivo. A “target” molecule is linked to a detector molecule (such as a protein or a nucleic acid) and the function and/or location of the target molecule is monitored by binding the detector molecule its ligand, which carries a detectable label. In particular embodiments of the invention, an intracellular fusion protein comprising a target protein and a detector protein is bound to a membrane-permeable, fluorescently-labeled ligand of the detector protein, thereby providing an adjunct or alternative to Green Fluorescent Protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.