Isolation and purification method of biomolecules using hydrogel
US7579151B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 27, 2005 |
| Grant date | Aug 25, 2009 |
| Priority date | — |
| Expiry date | Mar 11, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1006
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According to the method, the use of a hydrogel with a large surface area reduces the isolation time of biomolecules to 5 min or less, an external device such as an electromagnet is not required, and small-sized systems or LOC can be easily implemented due to applicability to microsystems through a polymer patterning technique.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.