Polypeptides and biosynthetic pathways for the production of stereoisomers of monatin and their precursors

US7582455B2 · kind B2 · utility

Assignee

• CARGILL, INCORPORATED · US

Inventors

• Brian Brazeau · Oskaloosa, US
• Ellen Burke · San Diego, US
• Mervyn Desouza · Plymouth, US
• Steven John Gort · Brooklyn Center, US
• Paula M. Hicks · Eden Prairie, US
• Sherry Kollmann · Maple Grove, US
• Peter Luginbuhl · San Diego, US
• Sara C. McFarlan · Windom, US
• Toby Richardson · San Diego, US
• Fernando A. Sanchez-Riera · South San Francisco, US
• Christopher Solheid · Minneapolis, US
• David Weiner · Echt, NL
• Lishan Zhao · Carlsbad, US

Key dates

• Filing date: Apr 26, 2006
• Grant date: Sep 1, 2009
• Expiry date: Nov 3, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/90
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.