Method for detecting kinase activity with thiol reactive fluorescent reagents
US7585643B2 · kind B2 · utility
7Cited by
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20Claims
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Key dates
| Filing date | Aug 30, 2006 |
| Grant date | Sep 8, 2009 |
| Priority date | — |
| Expiry date | Feb 8, 2027 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are assays useful for detecting kinase activity. The assays utilize thiol-reactive fluorescent reagents and include reacting a reaction mixture that contains an ATP analog such as ATPβS to form an ADP analog such as ADPβS if kinase activity is present in the reaction mixture. The ADP analog reacts with the thiol-reactive fluorescent reagent and the reagent exhibits a change in fluorescence, thereby indicating that kinase activity is present in the reaction mixture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.