Fluorescent probes for use in protein kinase inhibitor binding assay
US7588899B2 · kind B2 · utility
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1References
12Claims
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Key dates
| Filing date | Jul 25, 2006 |
| Grant date | Sep 15, 2009 |
| Priority date | — |
| Expiry date | Apr 29, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/02
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides methods relating to a screening assay format that can be applied to broad members of the protein kinase gene family. The assay uses a series of labeled, active site probes described herein that can be displaced by an inhibitor agent. The Kd for the inhibitor compound is derived based on the Kd of the probe for the kinase and the dose response of the inhibitor agent. The invention also provides active site probes suitable for use with the screening method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.