Method and compositions for ordering restriction fragments
US7598035B2 · kind B2 · utility
126Cited by
18References
5Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Oct 8, 2004 |
| Grant date | Oct 6, 2009 |
| Priority date | — |
| Expiry date | Dec 29, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a method for constructing a high resolution physical map of a polynucleotide. In accordance with the invention, nucleotide sequences are determined at the ends of restriction fragments produced by a plurality of digestions with a plurality of combinations of restriction endonucleases so that a pair of nucleotide sequences is obtained for each restriction fragment. A physical map of the polynucleotide is constructed by ordering the pairs of sequences by matching the identical sequences among the pairs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.