Composition using HMW dextran and method for in vitro preservation of corneal tissues
US7601487B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 6, 2006 |
| Grant date | Oct 13, 2009 |
| Priority date | — |
| Expiry date | Jan 20, 2027 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01N1/125
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
A corneal storage solution which maintains cell density and cell viability of corneas stored in vitro for a period of greater than four days up to about two weeks is provided. The solution contains a buffered, balanced, nutrient and electrolyte aqueous solution, at least one colloidal osmotic agent, a phenolic antioxidant compound, a non-lactate-generating substrate, a thiol-containing compound, insulin, and at least one antibiotic. A method for in vitro storage and preservation of the viability of human corneal endothelial cells for subsequent use includes the steps of removing a cornea from an eye globe and placing the cornea in the above corneal storage solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.