Recombinant methods for expressing a functional umami (T1R1/T1R3) taste receptor

US7601513B2 · kind B2 · utility

Assignee

• SENOMYX, INC. · US

Inventors

• Mark Zoller · San Diego, US
• Xiaodong Li · Bellevue, US
• Lena Staszewski · San Diego, US
• Shawn O'Connell · Encinitas, US
• Sergey Zozulya · San Diego, US
• Jon Elliot Adler · San Diego, US
• Hong Xu · San Diego, US
• Femando Echeverri · Chula Vista, US

Key dates

• Filing date: Dec 3, 2003
• Grant date: Oct 13, 2009
• Expiry date: Jun 4, 2026

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/04
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods for expressing a heteromeric taste receptor that responds to umami taste stimuli are provided. These methods comprise the co-expression of T1R1 and T1R3 nucleic acid sequences in a host cell that desirably also expresses a G protein that couples therewith, e.g., Gα15 and Gα16 or gustducin. In preferred embodiments, the host cells will be mammalian cells or Xenopus oocytes. These nucleic acid sequences are expressed constitutively or under inducible conditions. In preferred embodiments, the methods will yield mammalian cells that stably express a T1R1/T1R3 umami taste receptor under inducible conditions, e.g., HEK-293 cells that express Gα15.