Process of high purity albumin production
US7601515B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 23, 2007 |
| Grant date | Oct 13, 2009 |
| Priority date | — |
| Expiry date | Apr 23, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is provided for the preparation of albumin from a yeast culture medium which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, host proteins, fragments of albumin, polymers or aggregates of albumin and viruses, and which is essentially non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises separating the yeast from the culture medium to yield an albumin solution; subjecting the albumin solution to positive mode cation exchange, affinity, and positive mode anion exchange chromatography; and concentrating and formulating the albumin with sodium octanoate and sodium chloride.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.