Method of imaging fluorescence and fluorescence imaging apparatus
US7602494B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 5, 2007 |
| Grant date | Oct 13, 2009 |
| Priority date | — |
| Expiry date | Apr 17, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/367
- WIPO fieldOptics
- WIPO sectorInstruments
Abstract
A method of imaging fluorescence, in which an inverted fluorescence imaging apparatus provided with an immersion objective is used to image an observation phosphor for a constant term, includes an imaging position locating step of locating a vessel incorporating the observation phosphor at an imaging position of the fluorescence imaging apparatus; a liquid pouring step of pouring a preset amount of liquid between a top portion of the immersion objective in the fluorescence imaging apparatus and the vessel to bring about a charged state; an imaging step of imaging the observation phosphor in the vessel in a state where a space between the top portion of the immersion objective and the vessel is charged with the liquid; and a repeated control step of repeating a series of operations including the liquid pouring step of a single operation and the imaging step of a preset number of times of operations performed after the liquid pouring step of the single operation, at preset time intervals.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.