Patent · US Expired

Method of detection of classical swine fever

US7608433B2 · kind B2 · utility

0Cited by

29References

36Claims

0Family size

Assignee

IDEXX LABORATORIES INC. · US

Inventors

Bernd Hoffmann · Frankfurt am Main, DE
Klaus Depner · Neuenkirchen, DE
Martin Beer · Greifswald, DE

Key dates

Filing date	Feb 9, 2005
Grant date	Oct 27, 2009
Priority date	—
Expiry date	Mar 11, 2026

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/70
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a multiplex real-time RT-PCR assay with a heterologous internal control system (i.e., EGFP-RNA) for the simple and fast diagnosis of classical swine fever virus (CSFV). Primers and FAM-labeled TaqMan probes, specific for CSFV were selected by analyzing the consensus sequence of the 5′-non translated region of various CSFV strains. For determining the analytical sensitivity an in vitro transcript (T7-PC3Alf) of the 5′ NTR was constructed and tested. Furthermore, a primer-probe system for the detection of the internal control sequence was established, and a multiplex assay using CSF-System 1 and the IC real-time PCR could be performed as a one-tube assay without loss of sensitivity or specificity.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.