Pulse-medium perfusion bioreactor with improved mass transport for multiple-3-D cell constructs

US7608447B2 · kind B2 · utility

Assignee

• BEN GURION UNIVERSITY OF THE NEGEV RESEARCH AND DEVELOPMENT AUTHORITY · IL

Inventors

• Smadar Cohen · Beer Sheva, IL
• Tal Dvir · Nesher, IL
• Michal Shachar · Beer Sheva, IL

Key dates

• Filing date: Dec 9, 2004
• Grant date: Oct 27, 2009
• Expiry date: Dec 18, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M41/18
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed is a novel net member for supporting one or more cell constructs in the culture chamber of a bioreactor, which comprises an array of impermeable pyramidal elements protruding from the face thereof, wherein each of the corners of the base of each of said impermeable pyramidal elements comprises a circular opening. This net member provides for uniform and unobstructed flow of culture medium through 3D cell constructs in culture.Also disclosed are a bioreactor and bioreactor system incorporating said net member.The bioreactor and bioreactor system may be used in the bioproduction of therapeutic proteins and stem cell expansion.