Process for the extraction and isolation of insulin from recombinant sources
US7608583B2 · kind B2 · utility
6Cited by
1References
8Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 14, 2003 |
| Grant date | Oct 27, 2009 |
| Priority date | — |
| Expiry date | Sep 7, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/62
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the extraction and isolation of insulins from recombinant sources, particularly those expressed in and secreted by yeasts. Organic solvents have been used to extract host surface bound forms of insulin polypeptide. In addition, procedures for combining the steps medium clarification, solvent extraction and chromatography, in order to effect the simultaneous isolation and purification of soluble and membrane bound forms of insulin, is disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.