2-azapurine compounds and their uses

US7612187B2 · kind B2 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Frank Seela · Osnabrück, DE
• Helmut Rosemeyer · Osnabrück, DE
• Enno Schweinberger · Bodenfelde, DE
• Dieter Heindl · Munich, DE
• Frank Bergmann · Iffeldorf, DE

Key dates

• Filing date: May 25, 2006
• Grant date: Nov 3, 2009
• Expiry date: Jan 18, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6813
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

Within oligonucleotides 2-azapurine and especially 2-azaadenine bases form specifically base pairs with guanine. This base pair is of analogous stability as an adenine-thymine but less stable than a guanine-cytosine base pair. Therefore, the incorporation of 2-azaadenine residues into oligonucleotides instead of cytosine leads specifically to hybridization complexes with nucleic acids with homogenous stability. This is useful for the adaptation of the stabilities of different oligonucleotide sequences in all kinds of hybridization techniques, for example in oligomer chip technology.