Multiplex-bead complex for determination of lysosomal storage disorders

US7615224B2 · kind B2 · utility

Assignee

• Women's and Children's Hospital · AU

Inventors

• Peter John Meikle · Melbourne, AU
• John Joseph Hopwood · Adelaide, AU
• Douglas Alexander Brooks · Melbourne, AU
• Caroline Dean · McLaren Vale, AU

Key dates

• Filing date: Dec 1, 2005
• Grant date: Nov 10, 2009
• Expiry date: Dec 1, 2025

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/924
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Multiplexing bead technology is used for simultaneous screening of multiple LSD and normalizing measured enzyme activity or protein levels against other lysosomal proteins, enzymes, or enzyme activities. Diagnostic compositions include microspheres conjugated to purified antibodies that specifically bind LSD target antigens: saposin, LAMP-1, α-iduronidase, α-glucosidase, β-glucosidase, 2-sulphatase, 4-sulphatase, α-galactosidase, sphingomyelinase, 3-sulphatase or sulphamidase. The target antigens are naturally present in biological fluids or tissues of either LSD or non-LSD patients.