Process for producing extract for cell-free protein synthesis and cell extract produced thereby
US7615344B2 · kind B2 · utility
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Key dates
| Filing date | Nov 21, 2005 |
| Grant date | Nov 10, 2009 |
| Priority date | — |
| Expiry date | Jun 26, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
It is intended to provide a process for producing an extract for cell-free protein synthesis whereby the productivity of a protein and the production efficiency can be improved. Cells are cultured under suppressed growth conditions. In the stationary phase of the culture, the cells are collected and then disrupted. The above-described cells are preferably bacterial cells, in particular, Escherichia coli cells. In the suppressed growth conditions as described above, the culture temperature is preferably from 20 to 32° C., more preferably 26° C. or higher and lower than 30° C.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.