Bioinformatic method for identifying surface-anchored proteins from gram-positive bacteria and proteins obtained thereby
US7615616B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Sep 15, 2003 |
| Grant date | Nov 10, 2009 |
| Priority date | — |
| Expiry date | Jun 24, 2024 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A bioinformatic method is provided for identifying and isolating proteins with MSCRAMM®—like characteristics from Gram positive bacteria, such as Enterococcus, Staphylococcus, Streptococcus and Bacillus bacteria, which can then be utilized in methods to prevent and treat infections caused by Gram-positive bacteria. The method involves identifying from sequence information those proteins with a putative C-terminal LPXTG (SEQ ID NO:1) cell wall sorting signal and other structural similarities to MSCRAMM® proteins having the LPXTG-anchored cell wall proteins. The MSCRAMM® proteins and immunogenic regions therein that are identified and isolated using the present invention may be used to generate antibodies useful in the diagnosis, treatment or prevention of Gram positive bacterial infections.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.