Patent · US Expired

Method for purifying erythropoietin

US7619073B2 · kind B2 · utility

1Cited by

2References

16Claims

0Family size

Assignee

Bioceuticals Arzneimittel AG · DE

Inventors

Christof Schumann · Breitscheid, DE
Michael A. Mack · Bad Vilbel, DE
Jan-Ole Hesse · Bad Nauheim, DE

Key dates

Filing date	Jun 7, 2005
Grant date	Nov 17, 2009
Priority date	—
Expiry date	Jun 7, 2025

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K14/505
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a method for producing recombined erythropoietin having a particularly high degree of purity (≧98%). The method consists of at least 5 chromatographic purification steps, i.e. at least two anion exchange chromatographs, at least one hydrophobic interaction chromatography, at least one affinity chromatography and at least one hydroxyapatite chromatography. In preferred embodiments, the method does not require any exclusion chromatography and any reversed-phase chromatography. The invention relates to, in particular, a method wherein the following chromatographic purification steps have the following order; i) a first anion exchange chromatography, ii) an affinity chromatography, which relates, preferably, to a dye affinity chromatography, iii) a hydrophobic interaction chromatography, iv) an hydroxyapatite chromatography and v) a second anion exchange chromatography.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.