Ribozymes with broad tRNA aminoacylation activity

US7622248B2 · kind B2 · utility

Assignee

• The Research Foundation of the State University of New York · US

Inventors

• Hiroaki Suga · Tokyo, JP
• Hiroshi Murakami · Funabashi, JP
• Hirohide Saito · Kyoto, JP

Key dates

• Filing date: Feb 18, 2003
• Grant date: Nov 24, 2009
• Expiry date: Dec 26, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/12
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides catalytic RNA molecules having cis or trans aminoacylation activity. The catalytic RNA molecules having cis aminoacylation activity comprise a catalytic domain and an aminoacylation domain. The catalytic RNA molecules having trans aminoacylation activity only have the catalytic domain. A method is provided for constructing and screening of these molecules. These molecules are suitable for aminoacylating with specific amino acids.