Real-time gene quantification with internal standards
US7630837B2 · kind B2 · utility
7Cited by
17References
16Claims
0Family size
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Key dates
| Filing date | Aug 29, 2002 |
| Grant date | Dec 8, 2009 |
| Priority date | — |
| Expiry date | Jun 17, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/708
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a nucleic acid quantification kit and method for determining the initial concentration or mass fraction of a target nucleic acid present in a sample. Illustrative embodiments include real-time competitive quantitative polymerase chain reaction (PCR) to determine the copy number or mass fraction of a target nucleic acid sequence in a sample and use of a thermodynamically based signal processing algorithm, with or without PCR, to provide mass fraction information.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.