Method for microbially producing L-valine
US7632663B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 21, 2000 |
| Grant date | Dec 15, 2009 |
| Priority date | — |
| Expiry date | Feb 21, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P13/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a process for the microbial production of L-valine in which the dihydroxy acid-synthase (ilvD) activity and/or the ilvD gene expression is intensified in a microorganism. As an alternative or in combination with this, the acetohydroxy acid-synthase (ilvBN) activity and isomeroreductase (ilvC) activity and/or the ilvBNC gene expression are intensified in a microorganism. The process according to the invention preferably makes use of microorganisms in which the activity of at least one enzyme that is involved in a metabolic pathway that reduces the formation of L-valine is weakened or eliminated. Thus, for instance, the process according to the invention preferably makes use of microorganisms having a defect mutation in the threonine dehydratase (ilvA) gene and/or a defect mutation in one or more genes of the pantothenate synthesis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.