Modified nucleic acid sequences and methods for increasing mRNA levels and protein expression in cell systems
US7632980B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 20, 1998 |
| Grant date | Dec 15, 2009 |
| Priority date | — |
| Expiry date | Oct 20, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention provides modified recombinant nucleic acid sequences (preferably DNA) and methods for increasing the mRNA levels and protein expression of proteins which are known to be, or are likely to be, difficult to express in cell culture systems, mammalian cell culture systems, or in transgenic animals. The preferred “difficult” protein candidates for expression using the recombinant techniques of the invention are those proteins derived from heterologous cells preferably those of lower organisms such as parasites, bacteria, and virus, having DNA coding sequences comprising high overall AT content or AT rich regions and/or mRNA instability motifs and/or rare codons relative to the recombinant expression system to be used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.