Amplified signal in binding assays
US7635571B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 7, 2000 |
| Grant date | Dec 22, 2009 |
| Priority date | — |
| Expiry date | Jul 20, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/531
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods are disclosed for determining minute quantities of an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a substrate having an oxidant cleavable linker and a photosensitizer to come into close proximity. The photosensitizer generates singlet oxygen which oxidatively cleaves the linker to form a product which can be detected in a sandwich detection assay such as LOCI. The amount of product detected is related to the amount of analyte in the medium. Compositions, kits, and compounds are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.