Method to determine single nucleotide polymorphisms and mutations in nucleic acid sequence

US7638310B2 · kind B2 · utility

Assignee

• Estonian Biocenter · EE

Inventors

• Kaarel Krjutskov · Tartu, EE
• Andres Metspalu · Tartu, EE

Key dates

• Filing date: Jan 25, 2008
• Grant date: Dec 29, 2009
• Expiry date: Aug 27, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6827
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A genotyping method and a prepared oligomicroarray as device is to determine single nucleotide polymorphism (SNP) and mutations are provided. The method uses two specific APEX-2 primers per each SNP or mutations to be determined. The same primers are used in amplification phase (primer extension and PCR with universal primer) and in the single base extension phase on an array. All SNP-containing sequences can be genotyped and amplified in one reaction tube and visualized on a microarray.