Assay for the separation and quantification of hemagglutinin antigens
US7638608B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 3, 2005 |
| Grant date | Dec 29, 2009 |
| Priority date | — |
| Expiry date | Mar 3, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/12
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to novel methods for separating hemagglutinin (HA) antigens, comprising the steps of applying a reduced and derivatized antigen preparation comprising solubilized HA antigens and a detergent in a pH controlled solution, on a Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) column; and eluting the HA antigens from the column with an ion pairing agent in an organic mobile phase. The invention further relates to quantifying methods using the methods for separating the antigens with the further step of measuring the peak area of the eluted antigen in a chromatogram resulting from the elution step.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.