Method and device for PCR-amplification and detection of nucleotide sequences

US7642053B2 · kind B2 · utility

Assignee

• Siemens Aktiengesellschaft · DE

Inventors

• Walter Gumbrecht · Herzogenaurach, DE
• Manfred Stanzel · Erlangen, DE

Key dates

• Filing date: Dec 15, 2003
• Grant date: Jan 5, 2010
• Expiry date: Jul 26, 2024

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2300/0819
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A DNA-Chip includes a flat carrier and an array of spots containing probe molecules (oligonucleotides) which are arranged on said carrier. Each spot is associated with a microelectrode arrangement for impedance spectroscopic detection of binding events occurring between the probe molecules and target molecules (DNA fragments) applied by way of an analyte solution. In order to increase the sensitivity or the binding specific measuring effects of the biochip, the electrode arrangement is at least partially embedded in a hydrophilic reaction layer containing probe molecules and which is permeable to target molecules.