Enzyme-catalyzed metal deposition for the enhanced detection of analytes of interest
US7642064B2 · kind B2 · utility
11Cited by
32References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 20, 2004 |
| Grant date | Jan 5, 2010 |
| Priority date | — |
| Expiry date | Dec 8, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/581
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention is directed to enhanced methods for detecting an analyte of interest in situ, by immunoassay, or by hybridization comprising binding an enzyme-labeled conjugate molecule to an analyte of interest in the presence of a redox-inactive reductive species and a soluble metal ion. The enzyme catalyzes the conversion of the inactive reductive species to an active reducing agent, which in turn reduces the metal ion to a metal atom thereby providing an enhanced means of detecting the analyte via metal deposition.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.