Switchable photosystem-II designer algae for photobiological hydrogen production
US7642405B2 · kind B2 · utility
Inventor
Key dates
| Filing date | May 15, 2007 |
| Grant date | Jan 5, 2010 |
| Priority date | — |
| Expiry date | Aug 6, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/405
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A switchable photosystem-II designer algae for photobiological hydrogen production. The designer transgenic algae includes at least two transgenes for enhanced photobiological H2 production wherein a first transgene serves as a genetic switch that can controls photosystem II (PSII) oxygen evolution and a second transgene encodes for creation of free proton channels in the algal photosynthetic membrane. In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (302), a inducible promoter (304), a PSII-iRNA sequence (306), a terminator (308), and a PCR reverse primer (310). In other embodiments, the PSII-iRNA sequence (306) is replaced with a CF1-iRNA sequence (312), a streptomycin-production gene (314), a targeting sequence (316) followed by a proton-channel producing gene (318), or a PSII-producing gene (320). In one embodiment, a photo-bioreactor and gas-product separation and utilization system produce photobiological H2 from the switchable PSII designer alga.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.