Method for controlling sialylation of proteins produced by mammalian cells
US7645609B2 · kind B2 · utility
68Cited by
8References
28Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jul 15, 2003 |
| Grant date | Jan 12, 2010 |
| Priority date | — |
| Expiry date | Feb 15, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/005
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides media and methods for culturing mammalian cells whereby the sialylation of a protein produced by the cells is increased. The medium can contain N-acetylmannosamine and, optionally, galactose. The medium may also comprise fructose and mannose. Alternatively, the medium can contain galactose and fructose and, optionally, can also comprise mannose and/or N-acetylmannosamine. The methods can be practiced along with other methods for culturing cells so as to increase the quantity or quality of a protein produced by the cells, including culturing the cells at a temperature below 37° C.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.