Use of urea variants as affinity ligands
US7645903B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 12, 2003 |
| Grant date | Jan 12, 2010 |
| Priority date | — |
| Expiry date | Mar 20, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6854
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to an IgG-binding compound, which more specifically has affinity for human IgGs of κ-type and functional derivatives thereof. More specifically, the compound according to the invention comprises an N,N-alkylated urea moiety located between an aromatic part and another part, which is a linear or cyclic substituted or unsubstituted aliphatic group. The compound binds to a pocket-shaped binding site present on all human IgG κ-Fabs, which site is located between the two domains (CH1 and CL) of its constant part. Accordingly, the compound according to the invention is a ligand for human IgGs of κ-type, and consequently, the invention also relates to a separation matrix for affinity chromatography, which matrix comprises said compound, as well as to other uses of the compound.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.