Polyelectrolytic internal calibration system of a flow-through assay
US7651841B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 25, 2002 |
| Grant date | Jan 26, 2010 |
| Priority date | — |
| Expiry date | Dec 21, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54393
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A flow-through assay for detecting the quantity of an analyte residing in a test sample is provided. The flow-through assay contains a porous membrane that is in fluid communication with probe conjugates that contain a specific binding member and a detectable probe. The porous membrane also defines a detection zone and a calibration zone. The calibration zone contains a polyelectrolyte substantially non-diffusively immobilized on the porous membrane. The polyelectrolyte is capable of generating a detectable calibration signal that can be readily compared (visually, quantitatively, and the like) to a detection signal to determine the amount of analyte in the test sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.