Cultures of GFAP+ nestin+ cells that differentiate to neurons

US7651853B2 · kind B2 · utility

Assignee

• NsGene A/S · DK

Inventors

• Lars Ulrik Wahlberg
• Kenneth J. Campbell · Cincinnati, US
• Charlotta Skogh · Malmö, SE
• Cecilia Eriksson · Skanör med Falsterbo, SE
• Klas Wictorin · Lund, SE

Key dates

• Filing date: Oct 31, 2007
• Grant date: Jan 26, 2010
• Expiry date: Dec 10, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2501/11
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Cultures of cells immunoreactive for glial fibrillary acidic protein (GFAP), as well as for the intermediate filament marker nestin were grown in a medium including epidermal growth factor (EGF) and serum. The cultured cells had the morphology of astroglial cells. The cells can be proliferated in adherent or suspension cultures. Depending on the culture conditions, the cells can be induced to differentiate to neurons or glial cells. The cultures can be expanded over a large number of passages during several months, and survive, express an astroglial phenotype and integrate well after transplantation into both neonatal and adult rat forebrain.