Charge switch nucleotides

US7659070B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• John G. K. Williams · Ankeny, US
• Gregory R. Bashford · Lincoln, US
• Jiyan Chen · Lincoln, US
• Dan Draney · Lincoln, US
• Nara Narayanan · Greensboro, US
• Bambi L. Reynolds · Lincoln, US
• Pamela Sheaff · Omaha, US

Key dates

• Filing date: Oct 26, 2007
• Grant date: Feb 9, 2010
• Expiry date: Oct 26, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/00
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.