Method of separation using aromatic thioether ligands
US7659392B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 14, 2003 |
| Grant date | Feb 9, 2010 |
| Priority date | — |
| Expiry date | Feb 8, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/101
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is a method of separating nucleic acid molecules from contaminants, such as proteins, in a solution and isolating one or more desired nucleic acid molecules, which method comprises the following steps (a) providing an aqueous adsorption solution, which includes nucleic acids and a salt that forms lyotropic ions when dissolved; (b) passing said solution over a matrix to adsorb the nucleic acids onto the matrix, said matrix including an aromatic ring moiety and at least one thioether moiety; (c) passing an aqueous eluent over said matrix to desorb the nucleic acid molecules therefrom, which eluent includes a salt that forms lyotropic ions and a gradient of increasing ionic strength originating from an increasing concentration of a salt that forms less lyotropic ions when dissolved than the ones present in said aqueous adsorption solution; and (d) isolating a fraction comprising the desired nucleic acid molecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.