Methods for producing members of specific binding pairs
US7662557B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 18, 2004 |
| Grant date | Feb 16, 2010 |
| Priority date | — |
| Expiry date | Aug 15, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/735
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.