Compounds and methods for assessment of microsatellite instability (MSI) status

US7662595B2 · kind B2 · utility

Assignee

• MTM Laboratories, AG · DE

Inventors

• Peter Findeisen · Schriesheim, DE
• Sabine Merx · St. Leon-Rot, DE
• Matthias Kloor · Ludwigshafen, DE
• Magnus Von Knebel Doeberitz · Heidelberg, DE

Key dates

• Filing date: Oct 19, 2005
• Grant date: Feb 16, 2010
• Expiry date: Nov 21, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a method for assessment of the Microsatellite Instability (MSI) status of medically relevant conditions associated with MSI phenotype such as e.g. neoplastic lesions. The method is based on the analysis of a monomorphic T25 (CAT25) mononucleotide repeat located in the 3′-UTR of the Caspase 2 (CASP2) gene. Based on the determination of the length of the named mononucleotide repeat the presence or absence of MSI may be assessed. Determination of the length is performed in a single PCR procedure. Alternatively an enhanced assessment could be performed by combining the CAT25 marker with further markers such as BAT25 and BAT26 in a single multiplex PCR process.