Fluorescent detection method and reagent
US7662973B2 · kind B2 · utility
5Cited by
2References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 1, 2001 |
| Grant date | Feb 16, 2010 |
| Priority date | — |
| Expiry date | Jun 13, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/84
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed is a method for increasing the fluorescence of a Cyanine dye molecule comprising at least one NO2 group characterized by the reduction of the at least one NO2 group to NHOH or NH2 by the action of a nitroreductase. The cyanine dye molecule comprising at least one NO2 group can be used as a substrate for detecting nitroreductase enzyme activity in a composition and allows for the use of a nitroreductase enzyme in an enzyme-reporter system for the detection of analytes, binding reactions or gene expression.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.