P15 hairpin constructs and use
US7663024B2 · kind B2 · utility
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58Claims
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Key dates
| Filing date | May 3, 2006 |
| Grant date | Feb 16, 2010 |
| Priority date | — |
| Expiry date | Feb 22, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8283
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence. The invention further relates to genetically modified TGB-3 viral sequences suitable to induce gene silencing. In particular hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly the spread of the virus in the plant is significantly reduced or blocked.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.