Method for producing recombinant trypsin

US7666629B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Rainer Muller · Penzberg, DE
• Stephan Glaser · Seeshaupt, DE
• Frank Geipel · Penzberg, DE
• Johann-Peter Thalhofer · Weilheim, DE
• Bernhard Rexer · Weilheim, DE
• Claus Schneider · Eppelheim, DE
• Michael Ratka · Mannheim, DE
• Stephanie Ronning · Penzberg, DE
• Hellmut Eckstein · Weilheim, DE
• Claudia Giessel · Annaberg-Buchholz, DE

Key dates

• Filing date: Sep 11, 2007
• Grant date: Feb 23, 2010
• Expiry date: Sep 11, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/6427
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention concerns a method for producing recombinant trypsin from porcine pancreas in Pichia pastoris which is soluble and secreted into the culture medium, whereby expression at pH 3.0-4.0 substantially prevents activation of trypsinogen to β-trypsin and autolysis of β-trypsin by α-trypsin into ε-trypsin and from there into inactive peptides.