Green and orange fluorescent labels and their uses
US7667048B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 29, 2007 |
| Grant date | Feb 23, 2010 |
| Priority date | — |
| Expiry date | Dec 29, 2027 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/80
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides novel fluorescent compounds and covalent attachment chemistries which facilitate the use of these compounds as labels for ultrasensitive and quantitative fluorescent detection of low levels of biomolecules. In a preferred embodiment, the fluorescent labels of this invention are novel derivatives of the hydroxy-pyrene trisulphonic and disulphonic acids which may be used in any assay in which radioisotopes, colored dyes or other fluorescent molecules are currently used. Thus, for example, any assay using labeled antibodies, proteins, oligonucleotides or lipids, including fluorescent cell sorting, fluorescence microscopy (including dark-field microscopy), fluorescence polarization assays, ligand, receptor binding assays, receptor activation assays and diagnostic assays can benefit from use of the compounds disclosed herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.