Processes and vectors for amplification or expression of nucleic acid sequences of interest in plants

US7667092B2 · kind B2 · utility

Assignee

• Icon Genetics GmbH · DE

Inventors

• Victor Klimyuk · Leipzig, DE
• Yuri Gleba · Berlin, DE
• Mario Gils · Quedlinburg, DE
• Maxim V. Skulachev · Moskovskiy, RU
• Sylvestre Marillonet · Halle (Saale), DE

Key dates

• Filing date: Mar 27, 2002
• Grant date: Feb 23, 2010
• Expiry date: Jul 28, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2770/40022
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention discloses a biologically safe process of causing amplification and/or expression of one or more nucleic acid sequences of interest in a plant, plant tissue, plant cell or cell culture, characterized in that a plant cell is provided with at least two precursor vectors designed for undergoing processing by site-specific recombination in said cell, whereby due to said processing said plant cell is endowed with at least one replicon which provide(s) for said amplification and/or expression.