Design and selection of genetic targets for sequence resolved organism detection and identification
US7668664B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 22, 2007 |
| Grant date | Feb 23, 2010 |
| Priority date | — |
| Expiry date | May 30, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B25/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A computer-implemented method as follows. Providing a list of target sequences associated with one or more organisms. Providing a list of candidate prototype sequences suspected of hybridizing to one or more of the target sequences. Generating a collection of probes corresponding to each candidate prototype sequence, each collection of probes having a set of probes for every subsequence. The sets consist of the corresponding subsequence and every variation of the corresponding subsequence formed by varying a center nucleotide of the corresponding subsequence. Generating a set of fragments corresponding to each target sequence. Calculating the binding free energy of each fragment with a perfect complimentary sequence of the fragment. Determining which extended fragments are perfect matches to any of the probes. Assembling a base call sequence corresponding to each candidate prototype sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.