Surface plasmon resonance imaging of micro-arrays
US7670556B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 10, 2002 |
| Grant date | Mar 2, 2010 |
| Priority date | — |
| Expiry date | Nov 20, 2025 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/0636
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed is a method for fabricating 1-dimensional micro-arrays using parallel micro-fluidic channels on chemically-modified metal, carbon, silicon, and/or germanium surfaces; a μL detection volume method that uses 2-dimensional nucleic acid micro-arrays formed by employing the 1-dimensional DNA micro-arrays in conjunction with a second set of parallel micro-fluidic channels for solution delivery, and the 1-dimensional and 2-dimensional arrays used in the methods. The methodology allows the rapid creation of 1- and 2-dimensional arrays for SPR imaging and fluorescence imaging of DNA-DNA, DNA-RNA, DNA-protein, and protein-protein binding events. The invention enables very small volumes necessary for a variety of bioassay applications to be analyzed by SPR. Target solution volumes as small as 200 pL can be analyzed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.