Nucleotide mixture for improved nucleic acid amplification performance
US7670779B2 · kind B2 · utility
0Cited by
9References
9Claims
0Family size
Assignee
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Key dates
| Filing date | Feb 11, 2008 |
| Grant date | Mar 2, 2010 |
| Priority date | — |
| Expiry date | Feb 11, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to modification of amplification buffer used in amplification reactions. The modifications result in a significant improvement in results of amplification. In particular, the present invention provides methods and buffers for performing an amplification reaction utilizing a buffer comprising nucleotide triphosphates comprising treating the buffer to substitute a portion of the nucleotide triphosphates with nucleotide diphosphates.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.